Cepheid, sunnyvale, ca, a broad based molecular diagnostics company, announced the european release of the smart cmv cytomegalovirus assay for clinical diagnostic use on the smartcycler system. Confirmation of salmonella isolates by realtime fda. This type of reagent has several advantages including optimization and standardization of assay performance, less technical repetitive motion, less waste of materials, and less need for reagent storage space. Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting. Smart cycler instruments legal definition of smart. Prescriptionspecific parameters are entered into the cycler, which then automatically carries out the steps involved in continuous cycling pd. Apr 23, 2007 the smart hbvtm test developed and produced at our cepheid ab facility in stockholm extends and complements our existing line of products for monitoring of viral load in the management of transplant patients, said cepheid chief executive officer john bishop. Subject terms methods, realtime pcr, idaho technology, r. Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease. Jan 23, 2015 peritoneal dialysis pd is a widely accepted and increasingly popular form of dialysis.
Parameters involved in the conversion of realtime pcr assays. The reactions were initiated by addition of 1 l of sample. All pcr reactions were performed in 25l tubes cepheid in a smart cycler td system. Paul boschee director of corporate accounts 904 caribbean drive sunnyvale, ca 94089 paul. Computer is windows 2000 with cepheid smart cycler version 2. Evaluation of cepheid smartcycler and euroclone duplicareal time. Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Surplus property division only tests the units by plugging them up to see if they power on. The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. It is fdacleared for use in female endocervical swabs, patientcollected vaginal swabs and for female and. According to company literature, the icore module incorporates stateoftheart microfluidic and microelectronic design. Cepheid smart cycler 9000330 automated realtime pcr.
Eppendorf 5333 mastercycler pcr thermal cycler 96well. Chlamydia trachomatis, smartcycler, cepheid, euroclone, evaluation, realtime pcr introduction chlamydia trachomatis, a member of the chlamydiaceae demkin and zimin, 2005. Objective to compare the smartcycler ii system cepheid, sunnyvale, calif results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated polymerase chain reaction facility, and to establish the smartcycler ii system as a polymerase chain. Evaluation of the smartcycler ii system for realtime detection of. Review of use of a new rapid realtime pcr, the cepheid. The sc2000n31 is an addon block for the cepheid smartcycler ii realtime thermal cycler.
Download any file posted by another user of a community feature that you know, or reasonably should know, cannot be legally distributed in such manner or that you have a contractual obligation to keep confidential notwithstanding its availability on the web site. Methods genomic dna was extracted from whole blood. Unit is block only does not include any software or manuals. Cepheid smartcycler 16 site automated realtime pcr system. Cepheid enabling access to molecular diagnostic testing.
Contact cepheid customer care cepheid is available to support you online and by phone. Now you can pick the qpcr platform that best fits your research requirements find your fit today. Our it staff was able to power on the smart cycler and included laptop. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory. Its systems automate traditional laboratory tests for specific sequences of dna or rna. Cepheid smart cycler system authorized thermal cycler 1 serial. Cepheid s genexpert family of systems have set a new standard in workflow flexibility, 247 testing accuracy, and userfriendly designdelivered in an astonishingly beautiful and compact package. Cepheid smartcycler automated realtime pcr system thermal cycler 16 site. Each of the standard 16 icore modules expandable to 96 requires no warmup time, no routine maintenance, and no normalization dye. Cepheid sells clinical tests for healthcareassociated infections, infectious diseases, sexual health, oncology and genetics the cartridges used in. The cartridgebased assay detects dna of chlamydia trachomatis and neisseria gonorrhoeae. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and.
The assay is a rapid realtime pcr nucleic acid amplified test. The smart hbvtm test developed and produced at our cepheid ab facility in stockholm extends and complements our existing line of products for monitoring of viral load in the management of transplant patients, said cepheid chief executive officer john bishop. Smallpox and panorthopox virus detection by realtime 3. Pdf evaluation of the portable cepheid smartcycler realtime. This study evaluated the performance characteristics of the cepheid smartcycler used in conjunction with the euroclone duplicareal time dual easy ct assay for the confirmation of roche cobas taqman positive tests by comparing results obtained by each method. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. The test can provide rapid detection of the current pandemic coronavirus sarscov. Cepheid is an american molecular diagnostics company. Cepheid smart cycler ii td realtime pcr system govdeals. In 2006, cepheid introduced an even better way for any healthcare facility to leverage the power of molecular diagnostics. Mar 22, 2007 the smart hbvtm test developed and produced at our cepheid ab facility in stockholm extends and complements our existing line of products for monitoring of viral load in the management of transplant patients, said cepheid chief executive officer john bishop.
View and download king technology sparco cycler instruction manual online. The invention and technological advancement of the pd cycler further makes pd a convenient option. Evaluation of the cepheid smartcycler realtime pcr machine for rapid and portable diagnosis of. Expanding the boundaries for practical applications. Evaluation of the cepheid smartcycler realtime pcr machine for rapid and portable diagnosis of footandmouth disease find, read. As many as six of these blocks can be added to a single smartcycler unit, providing up to 96 independently controllable sites for read more. Cycler software free download cycler top 4 download. It is a molded plastic housing containing an immobilized submicrometer filter, lysis beads pdf on apr 1, 2002, a.
Cepheids smartcycler instrument is the only random access, modular real time. The paper from raja raja et al clinical chemistry 48. Cepheid smartcycler system software license agreement. Cycler software free download cycler top 4 download offers free software downloads for windows, mac, ios and android computers and mobile devices. Smartcycler ii pcr thermalcycler cepheid, sunnyvale, ca capable of performing cycling parameters described below and simultaneous realtime sequence detection for fam, tet, texas red and cy5 dyes. Smartcycler pcr reaction tubes minimum reaction volume of 25 l. Pcr instrument on the market with up to 96 sites that can each run a different. This block upgrades any existing smartcycler to accommodate an additional 16 reaction sites for real time pcr analysis. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing. Available realtime rtpcr cycler and kinetic pcr machines. Manufacturer specifications smartcycler ii td, cepheid please note.
Pdf evaluation of the portable cepheid smartcycler real. We pledge to offer the best service and warranty to all of our customers. Contribute to matplotlibcycler development by creating an account on github. Cepheid smartcycler ii thermal cycler automated realtime. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Cepheid and biomerieux enter into strategic commercial program. On this page the most prominent realtime pcr cycler are described. Evaluation of the cepheid smartcycler realtime pcr. Cepheid smartcycler ii td pcr manufacturer specifications. Used cepheid smartcycler ii thermal cycler automated real. Cepheid accessory for smartcycler pcr systems, cepheid.
Evaluation of the smartcycler ii system for realtime. Laboratory considerations for use of cepheid xpert mtbrif. Absolute blue qpcr mix, sybr green, with separate rox vial. Bob morrison gene expression pdf stlcccpls instrumentation specialist june 2008, latest revision feb 20 biorad model ptc1148 mj research model ptc150 cepheid smartcycler stratagene qpcr mx3005p hybaidpcr, omne mj research ptc220 biorad mycycler logon. Evaluation of the portable cepheid smartcycler realtime pcr machine for the rapid diagnosis of footandmouth disease by a hearps, z zhang and soren alexandersen cite. Xpert xpress sarscov2 cepheid has developed an automated molecular test for the qualitative detection of sarscov2, the virus that causes covid19. C rapid, precise heating and cooling of the reaction mixture to target temperatures significantly reduces cycling protocol tim.
What assay is optimal for the diagnosis of measles virus infection. Cepheid microdiagnostics systems cover the full range of biochemical processing required to yield an analytical result from sample collection, to. Detect fluorescence at each pcr cycle excite and detect famlabeled lux primers near their excitationemission wavelengths of 490520 nm, andor. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page. Sparco cycler swimming pool filter pdf manual download. Click on the smart cycler icon to start the life science 2d smartcycler software. Our genexpert family of systems have set a new standard in workflow flexibility, 247 testing accuracy, and userfriendly design delivered in an astonishingly beautiful and compact package the genexpert system is available in a one, two, four, or 16module configuration. Apr 11, 2006 the cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. The following outlines a rapid and robust method for detecting the human multidrug resistance gene mdr1 snp c3435t using real time polymerase chain reaction pcr. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif results.
Cepheid smartcycler system software license agreement license grant. The cepheid onepiece valve body combines filtration, wash, and lysis functions. Evaluation of cepheid smartcycler and euroclone duplicareal. Cphd today announced that it has entered into a strategic commercial relationship with biomerieux for biomerieux to develop dna testing products embodying its proprietary nasba nucleic acid sequencebased amplification technology to be run on systems employing cepheids smart cycler and genexpert platform technologies. Rapid and sensitive detection of mycobacterium dna using. The test leverages the design principles of our current xpert xpress flursv cartridge technology, in which multiple regions of the viral genome are targeted. Evaluation of the portable cepheid smartcycler realtime. Learn how to use adapter tray retainers in thermal cycler starter kit.
The laptop connects to the cycler and recognizes it. Cepheids smart cycler instrument facilitates the speed and accuracy of the idistrep b test. Cepheid our vision for a better way since the introduction of the smartcycler system in 2000, cepheid has been at the forefront of molecular diagnostics innovation. Click the button below to download a pdf of our latest catalog. The tests can be used to identify and analyze pathogens and genetic disorders. Cepheid introduces ce mark certified smart hbvtm test for. Realtime polymerase chain reaction for onehour onsite. Bsa per ml cepheid, and sterile molecular biology grade water to a total volume of 24 l. Cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. Cepheid smartcycler sc2 automated realtime pcr dell latitude i5 running windows 7 pro with smartcycler dx ver. Whether its customized environmental isolate controls, process controls, certified reference materials, proficiency samples, or something thats. To submit your question or concern, please click on contact support online and complete the form.
What assay is optimal for the diagnosis of measles virus. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. This is our way of saying thank you for allowing us to serve you. The cmv assay was released as a ce ivd mark product under the european directive on in vitro diagnostic medical devices. The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. All have our proven genexpert module at their analytic heart, and use the same patented cartridge technology for. Cepheid launches ce mark certification smart cmv in vitro. Evaluation of the portable cepheid smartcycler realtime pcr. The cepheid omnimix hslyophilized beads contain all of the reagents found in a master mix except primerprobe and target nucleic acid. Smartcycler icore module independently programmable unit. Available realtime pcr cyclers gene quantification. The heart of the smart cycler system from cepheid of sunnyvale, calif. Type ii cepheids are variable stars which pulsate with periods typically between 1 and 50 days.
The cepheid genexpert xpert ctng assay was us fdacleared in december 2012. Oct 01, 2001 rapid and sensitive detection of mycobacterium dna using cepheid smartcycler. We thrive on creating innovative biological reference materials that make microbiologists lives easier. Clinical chemistry, volume 47, issue 10, 1 october 2001, pages 19171918. Parameters involved in the conversion of realtime pcr. Cepheids genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. One of the pdf files decribes a comparison between lightcycler, smartcycler and abi on sensitivity. Objective to compare the smartcycler ii system cepheid, sunnyvale, calif results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated polymerase chain reaction facility, and to establish the smartcycler ii system as a polymerase chain reaction method for detecting viral and chlamydial dna from ocular. The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to newborns caused by the bacterium. View and download fisherprice smart cycle k5056 user manual online. Download user guide rest 2009 software user guide english pdf. Fisherprice smart cycle k5056 user manual pdf download. We designed, optimized, and extensively tested several sensitive and specific realtime pcr assays for rapid detection of both smallpox and pan orthopox virus dnas. Cepheid is expanding the boundaries for practical applications of microinstrumentation and microfluidics in the fields of biomedical analysis and diagnostics, especially for nucleic acid analysis.
Download any file posted by another user of a community feature that you know, or reasonably should know. King technology sparco cycler instruction manual pdf download. Evaluation of cepheid smartcycler and euroclone duplicareal time dual easy ct assay for confirmation of chlamydia. The smartcycler system at the heart of the smartcycler system is the icore module a reliable, solidstate optical system with no moving parts. By automating much of the testing process, and making each reaction site individually programmable, it is the fastest, easiest to use, and most flexible system now on the market. Cepheid xpert mtbrif assay november 20 3 all mtb isolates, including those that are rif sensitive, should receive additional dst by culturebased methods to determine the susceptibility patterns of other drugs used to treat tb. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. By automating the entire amplification and detection process, the smartcycler system can deliver highly accurate and consistent test results from prepared biological samples in 3040 minutes. An evaluation of the performance of a measles virus realtime reverse transcriptase pcr using the cepheid smartcycler.
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